The fate of Mycobacterium tuberculosis in activated human macrophages.
Vishwanath, V., Sujatha Narayanan, Narayanan, P.R.
Current Science, 1998, 75; 942-946.
Human peripheral blood monocytes, that are un-stimulated in in vitro , permit free multiplication of intra cellular mycobacterium tuberculosis after 72h in culture. There was no killing of bacilli in the intra cellular environment even after in vitro activation of monocytes with a cocktail of lipopolysaccharide, phorbol myristate acetate, interferon gamma and tumour necrosis factor-alpha. We also tested the ability of adenosine triphosphate (ATP) in reducing the intracellular viability of mycobacteria. Infected monocytes upon ATP treatment underwent cell death, but no loss in the intracellular viability of M.tuberculosis or M.smegmatis could be observed.
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