Cytochrome P-450 in drug-resistant Mycobacterium tuberculosis.
Geetha Ramachandran; Prema Gurumurthy; Narayanan, P.R.; Usha Mahadevan
Current Science; 1999; 76; 1231-1234.
Methods for the isolation and spectrophotometric determination of cytochrome P-450 in mycobacteria were standardized. Cytochrome P-450 levels were estimated in Mycobacterium tuberculosis organisms sensitive to both isoniazid and rifampicin and resistant to any of the two drugs. Cytochrome P-450 was isolated and its presence was shown in M.smegmatis, M.fortuitum, M.chelonae and M. tuberculosis H37Rv. The cytochrome P-450 content was significantly elevated in M. tuberculosis , resistant to both isoniazid and rifampicin when compared with the corresponding sensitive strains. It therefore appears that cytochrome P-450 might play a role in causing drug resistance in tuberculosis.
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