Evaluation of PCR using TRC 4 and IS 6110 primers in detection of tuberculous meningitis.

Sujatha Narayanan; Vijayalakshmi Parandaman; Narayanan, P.R.; Venkatesan, P.; Girish, C.; Mahadevan, S.; Sarala Rajaji

Journal of Clinical Microbiology; 2001; 39; 2006-2008.          

We have evaluated a new set of primers (TRC 4 ) in comparison with the IS 6110 primers commonly used in PCR to detect tuberculous meningitis among children. The levels of concordance between the results of IS 6110 PCR and TRC 4 PCR with cerebrospinal fluid specimens from patients with clinically confirmed tuberculous meningitis were 80 and 86%, respectively. Results with the two primer sets were concordant for 55 positive and 22 negative specimens ( n =98). We conclude that the sensitivity of PCR can be increased by using both IS 6110 and TRC 4 primers.

Back to List of publications / Home