Purification of cytochrome P-450 in mycobacteria.

Geetha Ramachandran; Prema Gurumurthy.

Current Science; 2002; 83; 873-877.

Purification of cytochrome P-450 from Mycobacterium smegmatis , M. chelonae , M. fortuitum and M. tuberculosis H37Rv was undertaken. The electrophoretic pattern revealed a single band corresponding to a molecular weight of 66 kDa in all the four species. Cytochrome P-450 purified from drug-resistant M. tuberculosis showed a different pattern from that of the sensitive bacteria and the former was similar to the purified product obtained from phenobarbital-induced cytochrome P-450 in M. tuberculosis H37Rv. It therefore appears that different forms of cytochrome P-450 are present in drug-sensitive and resistant M. tuberculosis and that there is similarity in the pattern between drug-resistant and Phenobarbital-induced M. tuberculosis.

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