CCR2, MCP-1, SDF-1α & DC-sign gene polymorphisms in HIV-1 infected patients with & without tuberculosis.

Alagarasu, K.; Selvaraj, P.; Swaminathan, S.; Raghavan, S.; Narendran, G.; Narayanan, P.R.

Indian Journal of Medical Research; 2009; 130; 444-450.

Background & objectives : Variability in the clinical outcome of persons exposed to and infected with HIV-1 and tuberculosis (TB) is determined by multiple factors including host genetic variations. The aim of the present study was to find out whether chemokine, chemokine receptor and DC-SIGN gene polymorphisms were associated with susceptibility or resistance to HIV and HIV-TB in south India .

Methods : CCR2 V64I (G/A), monocyte chemoattractant protein-1 (MCP-1) -2518 A/G, stromal cell derived factor-1α (SDF-1α) 3'UTR G/A and DC-SIGN gene polymorphisms were studied by polymerase chain reaction based methods in HIV-1 infected patients without TB (n=151), with pulmonary TB (PTB) (n=81) and extrapulmonary TB (n=31), 155 PTB patients without HIV and 206 healthy controls.

Results : The genotype frequencies of CCR2 V64I, MCP-1 -2518 and DC-SIGN polymorphisms did not differ significantly between the study groups. A significantly increased frequency of GG genotype of SDF-1α polymorphism was observed among HIV+PTB+ patients compared to healthy controls ( P =0.009, Pc=0.027).

Interpretation & conclusions : Our data suggest that GG genotype of SDF-1α 3'UTR polymorphism may be associated with susceptibility to PTB in HIV-1 infected patients. A better understanding of genetic factors that are associated with TB could help target preventive strategies to those HIV patients likely to develop tuberculosis.

Keywords: Chemokine - chemokine receptor - gene polymorphisms - HIV - HIV-TB

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