Haplotype analysis of HLA-A, -B antigens and -DRB1 alleles in south Indian HIV-1-infected patients with and without pulmonary tuberculosis.

Raghavan, S.; Selvaraj, P.; Swaminathan, S.; Alagarasu, K.; Narendran, G.; Narayanan , P.R.

International Journal of Immunogenetics; 2009; 36; 129-133.

Summary: We have shown earlier the association of human leucocyte antigen (HLA)-A11 with resistance and HLA-B40 and -DR2 with susceptibility to HIV and HIV-TB. In the present study, we have attempted to find out the HLA-DR2 subtypes and the possible HLA-A/-B/-DRB1 haplotype combinations that are associated with susceptibility or resistance to HIV and HIV with pulmonary tuberculosis (HIV+PTB+). HLA-DR2 subtyping was carried out by polymerase chain reaction-based sequence-specific oligonucleotide probe method. Overrepresentation of HLA-DRB1*1501 in HIV-positive PTB-negative (HIV+PTB–) patients ( P =0.004, P c =0.06) and -DRB1*1502 in HIV-positive PTB-positive (HIV+PTB+) patients ( P =0.019) was observed as compared to healthy controls. Haplotype analysis revealed an increased frequency of HLA-A2-DRB1*1501 haplotype in HIV+PTB– patients ( P =0.008) and HLA-A2-DRB1*1502 among HIV+PTB+ patients ( P =0.01) compared to healthy controls. The haplotypes B40-DRB1*1501 and B40-DRB1*04 were found to be moderately increased in HIV +PTB– and HIV+PTB+ patients ( P <0.05). The study suggests that HLA-A2-DRB1*1501 haplotype may be associated with HIV infection while HLAA2-DRB1*1502 haplotype might be associated with susceptibility to PTB in HIV patients. Moreover, HLAB40-DRB1*1501 and HLA-B40-DRB1*04 haplotypes may be associated with susceptibility to HIV infection and to PTB in HIV patients.

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