Simple and rapid liquid chromatography method for determination of moxifloxacin in plasma.

Hemanth Kumar, A.K.; Geetha Ramachandran , G.

Journal of Chromatography B; 2009; 877; 1205–1208.

Abstract: A high performance liquid chromatographic method for determination of moxifloxacin in human plasma was developed. The method involved deproteinisation of the sample with perchloric acid and analysis of the supernatant using a reversed-phase C 18 column (150mm) and fluorescence detection at an excitation wavelength of 290nm and an emission wavelength of 460 nm. The assay was specific for moxifloxacin and linear from 0.125 to 10.0 m g/ml. The relative standard deviation of intra- and inter-day assays was lower than 10%. The average recovery of moxifloxacin from plasma was 101%. Due to its simplicity, the assay can be used for pharmacokinetic studies of moxifloxacin.

Keywords: Moxifloxacin – Plasma - HPLC

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