Effect of bacteriophage lysin on lysogens.

Subramanyam, B.; Kumar,V.

Asian Pacific Journal of Tropical Biomedicine; 2011; 1; 306-308.      
   

Abstract: Objective: To study the effect of phage lysine on the growth of lysogens.

Methods: Sputum specimens processed by modified Petroff's method were respectively treated with phagebiotics in combination with lysine and lysine alone. The specimens were incubated at 37^o C for 4 days. At the end of day 1, 2, 3 and day 4, the specimens were streaked on blood agar plates and incubated at 37^o C for 18-24 hours. The growth of normal flora observed after day 1 was considered as lysogens.

Results: Sputum specimens treated with phagebiotics-lysin showed the growth of lysogens. When specimens treated with lysine alone, lysogen formation was avoided and normal flora was controlled.

Conclusions: Lysin may have no effect on the growth of lysogens.

Keywords: Bacteriophages; Phage lysine; Normal flora

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