T-h-2 immunity and CD3 + CD45RB low -activated T cells inmice immunized with recombinant bacillus Calmette-Guerin expressing HIV-1 principal neutralizing determinant epitope.

Aravindhan, V.; Sujatha Narayanan; Gautham, N.; Prasad, V.; Kannan, P.; Jacobs Jr. W.R.; Narayanan, P.R.

FEMS Immunology and Medical Microbiology; 2006; 47; 45-55.

Abstract: The genetic engineering of Mycobacterium bovis –bacillus Calmette–Guerin to express foreign epitopes is an attractive strategy in the field of epitope vaccines. We constructed an ‘epitope-trap vector' with Mycobacterium tuberculosis chaperonin-10 as a carrier antigen and used it to express the HIV-1 principal neutralizing determinant epitope. We also identified a new chaperonin-10 promoter that was hyperexpressive compared with the heat shock protein-65 promoter. Splenocytes from recombinant bacillus Calmette–Guerin-immunized mice showed enhanced lymphocyte proliferation and interleukin-4 (but not interferon- g ) secretion. The recombinant bacillus Calmette–Guerin-immunized group also exhibited mild delayed-type hypersensitivity reaction and a high frequency of CD3 + CD45RB low -activated T cells, together with high titer of antiprincipal neutralizing determinant immunoglobulin G antibodies. Thus, this epitope delivery system induced strong epitope-specific T-h-2 polarization.

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