Simple and rapid liquid chromatography method for determination of efavirenz in plasma.

Ramachandran, G.; Kumar, A.K.; Swaminathan, S.; Venkatesan, P.; Kumaraswami, V.; Greenblatt, D.J.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences; 2006; 835; 131-135.

Abstract : A simple and rapid high performance liquid chromatographic method for determination of efavirenz in human plasma was developed. The methd involved extraction of sample with ethyl acetate and analysis using a reversed-phase C 18 column (150mm) with UV detection. The assay was linear from 0.0625 to 10.0 m g/ml. The method was specific for efavirenz estimation and the drug was stable in plasma up to one month at -20 o C. The average recovery of efavirenz from plasma was 101%. Due to its simplicity, the assay can be used for pharmacokinetic studies and therapeutic drug monitoring of efavirenz.

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