1, 25-dihydroxyvitamin D3 downregulates cytotoxic effector response in pulmonary tuberculosis.

Afsal, K.; Selvaraj, P.; Harishankar, M.

International Immunopharmacology; 2018; 62: 251-260.

Abstract: 1,25-dihydroxyvitaminD3 [1,25(OH)2D3] modulates both the innate and adaptive immunity in tuberculosis. We explored the e?ect of 1,25(OH)2D3 on cytolytic molecules like perforin, granulysin, and granzyme-B in T-cells and natural killer cells during M. tuberculosis (Mtb) infection. Peripheral blood mononuclear cells (PBMCs) from 45 healthy controls (HCs) and 45 pulmonary tuberculosis (PTB) patients were cultured with Mtb in the absence or presence of 1,25(OH)2D3 for 72h. The percentage of perforin, granulysin, and granzyme-B positive cells were estimated by ?ow cytometry. 1,25(OH)2D3 signi?cantly decreased the percentage of cytolytic molecules in total, CD4+, CD8+ and CD56+ cells in HCs and PTB patients (p < 0.05). Moreover, 1,25(OH)2D3 downregulates IFN-? levels while upregulate the anti-in?ammatory cytokine IL-10. Correlation revealed that the total percentage of cytolytic molecules were positively correlated with IFN-? level, whereas negatively correlated withIL10 level in both the study subjects (p < 0.05). This results suggests that 1,25(OH)2D3 downregulate the expression of cytolytic molecues and act as anti-in?ammatory in adaptive immune response, which might help to reduce in?ammation and tissue damage during the active stage of the disease.

Keywords : Tuberculosis; Vitamin D; Cytolytic molecules; Cytokine; In?ammation  

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